ICCS2004 Application Submission/Review

Abstract for
"FLUORESCENCE IMAGING FOR COMPLEX DYNAMIC INTERACTIONS OF ORGANELLES IN LIVING CELLS"


Fluorescence imaging for the study of organelle interactions in living normal and pathological cells uses selective vital probes: dimethylaminostyrylpyridiniummethyliodine and tetramethyl- rhodaminemethylester for mitochondriaal visualization and membrane potential, pyrenebutylrhodamine for mitochondrial oxygen, NBD ceramide for the Golgi apparatus, dihexyloxacarbo- cyanine idodide for the endoplasmic reticulum, atebrine and the fluorogenic probe nonylumbelliferylglucoside for the lysosomes. Designs of Fourier interferometry based on Michelson, Sagnac interferometers or a Pentaferometer are being introduced for improved fluorecence excitation/emission spectral imaging. Combinedfluorescence/photoacoustic imaging is proposed to also account for the component of the excitation energy going into internal coversion. Biomedical applications have been initiated on a variety of cancer cells including colon and breast, with potential significance for diagnostics, prognostics and dru trials. There are also biotechnological applications on organelles of hydrogen-producing algae, e.g. Chlamydomonas.