"FLUORESCENCE IMAGING FOR COMPLEX DYNAMIC INTERACTIONS OF ORGANELLES IN LIVING CELLS"
Fluorescence imaging for the study of organelle interactions in living normal and pathological cells uses selective vital probes: dimethylaminostyrylpyridiniummethyliodine and tetramethyl- rhodaminemethylester for mitochondriaal visualization and membrane potential, pyrenebutylrhodamine for mitochondrial oxygen, NBD ceramide for the Golgi apparatus, dihexyloxacarbo- cyanine idodide for the endoplasmic reticulum, atebrine and the fluorogenic probe nonylumbelliferylglucoside for the lysosomes. Designs of Fourier interferometry based on Michelson, Sagnac interferometers or a Pentaferometer are being introduced for improved fluorecence excitation/emission spectral imaging. Combinedfluorescence/photoacoustic imaging is proposed to also account for the component of the excitation energy going into internal coversion. Biomedical applications have been initiated on a variety of cancer cells including colon and breast, with potential significance for diagnostics, prognostics and dru trials. There are also biotechnological applications on organelles of hydrogen-producing algae, e.g. Chlamydomonas.